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The Knipe laboratory studies mechanisms of lung diseases such as acute lung injury and pulmonary fibrosis. Our lab is especially interested in the role of the endothelium in the development of pulmonary fibrosis and conducts several projects involving both translational human studies and mouse models of disease. The individual hired for this position will have some experience with basic cell biology techniques (ideally gene expression by PCR and protein expression by Western blotting) and a high degree of computer literacy. This person will be involved in translational human and mouse studies using a variety of molecular and cellular techniques including, but not limited to: flow cytometry, single-cell sorting, DNA and RNA extraction, RNA sequencing, PCR and real-time PCR analysis, cell isolation and culture, and immunohistochemistry. This person will also assist with mouse studies, including disease modeling, surgery/procedures, husbandry, and cell culture. There will also be opportunities to help collect and process human lung tissue from lung transplantations to translate into various culture studies in the lab, which will require interfacing with MGH pathology. This position is an excellent opportunity to work with a collaborative and collegial group of lung biology researchers focuses on developing novel anti-fibrotic therapies.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
Please note, the functions below are representative of major duties that are typically associated with these positions. Specific responsibilities may vary based upon departmental needs. Similarly, not all duties that have been outlined will be assigned to each position.
A Research Technician II performs the duties of a Research Technician I (above) and may also:
SKILLS/ABILITIES/COMPETENCIES REQUIRED:
EDUCATION:
EXPERIENCE:
SUPERVISORY RESPONSIBILITY (if applicable):
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